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cd45 microbeads, mouse  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd45 microbeads, mouse
    Cd45 Microbeads, Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 961 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd45+microbeads/CD45+MicroBeads%2C+mouse/custom%40130-052-301%4042635130
    Average 97 stars, based on 961 article reviews
    cd45 microbeads, mouse - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Single Cell:

    Article Title: Preliminary Analysis of the Proportion and Characteristics of Dual BCR B Cells in SLE Model Mice and Patients via scRNA-Seq Combined with scBCR-Seq Technology
    Article Snippet: .. From the prepared single-cell suspension, CD45-positive cells were isolated from 10 7 splenocytes per sample using CD45 MicroBeads (Miltenyi Biotec, Cologne, Germany). ..

    Article Title: Decoding cellular communication networks and signaling pathways in bone, skeletal muscle, and bone-muscle crosstalk through spatial transcriptomics in a young male mouse.
    Article Snippet: .. Human bone single-cell suspensions were prepared from femoral head biopsies following a published protocol.121 Briefly, bone fragments (0.5–1 cm3) were digested twice with type II collagenase (1mg/mL, 37 °C, 30 + 60min), filtered (70 μm), treated with RBC lysis buffer, and depleted of hematopoietic (CD45+) cells using CD45 MicroBeads (Miltenyi Biotec). .. Cell suspensions (>70% viability) were processed on the 10× Genomics Chromium platform with the Chromium Fixed RNA Kit and sequenced on Illumina NextSeq 2000 (P3 100-cycle kit; ~6 500 cells/sample, ~50 000 reads/cell).

    Suspension:

    Article Title: Preliminary Analysis of the Proportion and Characteristics of Dual BCR B Cells in SLE Model Mice and Patients via scRNA-Seq Combined with scBCR-Seq Technology
    Article Snippet: .. From the prepared single-cell suspension, CD45-positive cells were isolated from 10 7 splenocytes per sample using CD45 MicroBeads (Miltenyi Biotec, Cologne, Germany). ..

    Article Title: CXCL9/SPP1-polarized tumor-associated macrophages exert dual roles in regulating anti-tumor immunity in lung adenocarcinoma.
    Article Snippet: .. The cell suspension was mixed with CD45 MicroBeads, mouse (Miltenyi Biotec, 130-052-301, Germany) at the ratio specified in the product manual, incubated at 4 °C for 15 min, and subjected to positive selection using the MACS magnetic column system to collect CD45+ tumor immune cells. .. Macrophages were further enriched from CD45+ cells using F4/80 MicroBeads, mouse (Miltenyi Biotec, 130-110-443, Germany) for magnetic sorting to obtain F4/80+ macrophages.

    Isolation:

    Article Title: Preliminary Analysis of the Proportion and Characteristics of Dual BCR B Cells in SLE Model Mice and Patients via scRNA-Seq Combined with scBCR-Seq Technology
    Article Snippet: .. From the prepared single-cell suspension, CD45-positive cells were isolated from 10 7 splenocytes per sample using CD45 MicroBeads (Miltenyi Biotec, Cologne, Germany). ..

    FACS:

    Article Title: From paradox to target: IFN-β hijacks MEK signaling to drive a cell death-evading dormant phenotype in colorectal cancer.
    Article Snippet: .. Upon tumor harvesting and tissue dissociation, total tumor-infiltrating immune cells were first enriched by isolating CD45+ cells via magnetic-activated cell sorting (MACS) using CD45 MicroBeads (Miltenyi Biotec) according to the manufacturer's protocol. ..

    Magnetic Cell Separation:

    Article Title: From paradox to target: IFN-β hijacks MEK signaling to drive a cell death-evading dormant phenotype in colorectal cancer.
    Article Snippet: .. Upon tumor harvesting and tissue dissociation, total tumor-infiltrating immune cells were first enriched by isolating CD45+ cells via magnetic-activated cell sorting (MACS) using CD45 MicroBeads (Miltenyi Biotec) according to the manufacturer's protocol. ..

    Article Title: CXCL9/SPP1-polarized tumor-associated macrophages exert dual roles in regulating anti-tumor immunity in lung adenocarcinoma.
    Article Snippet: .. The cell suspension was mixed with CD45 MicroBeads, mouse (Miltenyi Biotec, 130-052-301, Germany) at the ratio specified in the product manual, incubated at 4 °C for 15 min, and subjected to positive selection using the MACS magnetic column system to collect CD45+ tumor immune cells. .. Macrophages were further enriched from CD45+ cells using F4/80 MicroBeads, mouse (Miltenyi Biotec, 130-110-443, Germany) for magnetic sorting to obtain F4/80+ macrophages.

    Article Title: IDH2 deficiency enhances ferroptosis-induced vascular inflammation and remodeling in diabetes.
    Article Snippet: .. CD31 MicroBeads (cat no. 130-097-418) and CD45 MicroBeads (cat no. 130-052-301) used for MACS were obtained from Miltenyi Biotec, Inc. (Bergisch Gladbach, Germany). ..

    Incubation:

    Article Title: CXCL9/SPP1-polarized tumor-associated macrophages exert dual roles in regulating anti-tumor immunity in lung adenocarcinoma.
    Article Snippet: .. The cell suspension was mixed with CD45 MicroBeads, mouse (Miltenyi Biotec, 130-052-301, Germany) at the ratio specified in the product manual, incubated at 4 °C for 15 min, and subjected to positive selection using the MACS magnetic column system to collect CD45+ tumor immune cells. .. Macrophages were further enriched from CD45+ cells using F4/80 MicroBeads, mouse (Miltenyi Biotec, 130-110-443, Germany) for magnetic sorting to obtain F4/80+ macrophages.

    Selection:

    Article Title: CXCL9/SPP1-polarized tumor-associated macrophages exert dual roles in regulating anti-tumor immunity in lung adenocarcinoma.
    Article Snippet: .. The cell suspension was mixed with CD45 MicroBeads, mouse (Miltenyi Biotec, 130-052-301, Germany) at the ratio specified in the product manual, incubated at 4 °C for 15 min, and subjected to positive selection using the MACS magnetic column system to collect CD45+ tumor immune cells. .. Macrophages were further enriched from CD45+ cells using F4/80 MicroBeads, mouse (Miltenyi Biotec, 130-110-443, Germany) for magnetic sorting to obtain F4/80+ macrophages.

    Red Blood Cell Lysis:

    Article Title: Decoding cellular communication networks and signaling pathways in bone, skeletal muscle, and bone-muscle crosstalk through spatial transcriptomics in a young male mouse.
    Article Snippet: .. Human bone single-cell suspensions were prepared from femoral head biopsies following a published protocol.121 Briefly, bone fragments (0.5–1 cm3) were digested twice with type II collagenase (1mg/mL, 37 °C, 30 + 60min), filtered (70 μm), treated with RBC lysis buffer, and depleted of hematopoietic (CD45+) cells using CD45 MicroBeads (Miltenyi Biotec). .. Cell suspensions (>70% viability) were processed on the 10× Genomics Chromium platform with the Chromium Fixed RNA Kit and sequenced on Illumina NextSeq 2000 (P3 100-cycle kit; ~6 500 cells/sample, ~50 000 reads/cell).

    Article Title: Decoding cellular communication networks and signaling pathways in bone, skeletal muscle, and bone-muscle crosstalk through spatial transcriptomics in a young male mouse
    Article Snippet: .. Briefly, bone fragments (0.5–1 cm ) were digested twice with type II collagenase (1 mg/mL, 37 °C, 30 + 60 min), filtered (70 μm), treated with RBC lysis buffer, and depleted of hematopoietic (CD45 + ) cells using CD45 MicroBeads (Miltenyi Biotec). .. Cell suspensions (>70% viability) were processed on the 10× Genomics Chromium platform with the Chromium Fixed RNA Kit and sequenced on Illumina NextSeq 2000 (P3 100-cycle kit; ~6 500 cells/sample, ~50 000 reads/cell).



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